Background: Auditory reaction time (ART) and visual reaction time (VRT) are non-invasive techniques used to assess the capability of the CNS to integrate sensory and motor activities based on the level of CNS arousal and alertness. There is a lacuna for studying the impact of nicotine in smokeless tobacco users, therefore this study aimed to estimate and compare ART and VRT of smokers and dipping tobacco users with non-tobacco users in different age groups. Methods: Total 612 apparently healthy male subjects were divided equally on the basis of habit into three groups namely smokers, dipping tobacco users and control (non- tobacco user) with 204 subjects in each group. They were divided further on the basis of age into 4 sub groups like 11-20 years, 21-30 years, 31-40 years and 41-50 years with having 51 subjects in each group. RTM-608 (Medicaid system) apparatus was used to measure audio-visual reaction time under strict precautions and standard protocols to minimize the effect of other variables on reaction times. Data was analyzed using one way analysis of variance in each group. Results: All age groups differ significantly from each other for ART and VRT (ANOVA; p<0.05). Significant reduction was found for ART & VRT for dipping tobacco users as compared to control and smokers (p <0.05). ART and VRT increases with age in each group of control, smokers and dipping tobacco users. Conclusion: The audio and visual reaction time was significantly higher in older individuals. Reduction in ART and VRT were observed in the dipping tobacco users and smoker which shows acceleration of the response to stimulus