HPV being the most important risk factor, needs to be evaluated in all the regions of the country since the prevalence of HPV and its genotypes are different in the populations from different geographical regions. Its assessment at various stages of the disease will also be helpful in understanding its role in cervical cancer pathogenesis. The present series of work entitled as “Study of cytodiagnosis of discharge per vaginum with specific reference to HPV and HSV at Index Medical College, Indore” was undertaken to detect cervical lesions of female genital tract and prevalence of HPV and HSV by means of exfoliative cytology. Out of 1542, 9 cases showed cytomorphological features of HPV and 2 cases showed cytomorphological features of HSV. All 11 cases were found to be associated with multiple risk factors. In this study, epithelial cell abnormality are more common in hindu (85.19%). In muslims, it is only (14.81%).