Introduction: Type 2 diabetes mellitus (T2DM) is inherited genetically. There may be an association with blood group as blood group is predetermined genetically. Methods: Two hundred and thirty one (231) diabetic patients are included in the study which conducted by department of physiology. Diabetic patients were taken from medicine department, and the distribution of blood group among apparently healthy blood donors at Department of Immunohematology and Blood Transfusion (IHBT) was taken as control population. Results: Our study showed no statistically significant association of T2DM with blood group (p-value=0.81). The percentages of blood group O, A, B and AB in T2DM and control are respectively (31.16% & 34%) (30.73% & 32%), (25.10% & 23%) and (12.98% & 9%).Conclusion: The present study showed no significant association of ABO blood groups with T2DM.