The aim of this study was to determine critical predictive serum bilirubin value on first day (24 hrs) of life which can screen healthy term newborns at risk of developing significant hyperbilirubinemia. This was a prospective study conducted at "Maharaja Yashwant Rao Hospital Indore" between August 2008 and September 2009. Serum bilirubin estimation was done by Van Den Berg method to know total and direct reacting bilirubin. We believe that data from this study could be applied to babies at low risk and specially will help to screen babies who may develop neonatal hyperbilirubinemia (NNHB) inspite ruling out risk factors. This group mainly represent in which hyperbilirubinemia is due to- increased enterohepatic circulation, breast milk jaundice, hypothyroidism, drugs, galactosemia, Crigler-Najjar syndrome, Gilbert syndrome, infection etc.