Food borne diseases are the major health problems in developing countries like India. The problem is more noticeable due to prevailing poor food handling and sanitation practices. Street food vending has been increasing for several years but this growth presents public health challenge with evidence of diarrheal diseases. Emerging needs for understanding the food hygiene knowledge of street food vendors to ensure hygienic preparation of street foods is essential. The purpose of this study is to assess the level of knowledge on food hygiene among street food vendors in urban Chidambaram. The study conducted using a descriptive cross-sectional survey. A total of 50 street food vendors were selected using purposive sampling technique. Data were collected by conducting face to face interview using a semistructured questionnaire. The questionnaire was peer-reviewed and pilot tested before the final version. The questionnaire were structured into three distinctive parts to collect information on demographic characteristics, personal hygiene and knowledge on food safety .Among the study participants majority were of the age group 20 to 29 yrs ie 25[50%] majority of the participants were males 35[70%] and 33[66%] were married. With regards to cooking process 44[88%] of participants had moderate knowledge with mean score of 59.66±12.63. Knowledge about diseases was also adequate among 46[92%] participants with mean score of 97.33±9.13. .The study shows the overall knowledge level on food hygiene among street food vendors were adequate with 82.63±6.40. The factors like age, gender, education, type of shop or years of experience had no significant association with the knowledge levels of the participants. To conclude, Food vendors should be adequately educated on the role of food in disease transmission as well as on rules of personal hygiene and approved practices in handling street food