Introduction: Several plants have been proven useful as antioxidant agents as a result of the abundance of bioactive substances embedded in the plant. Eugenia uniflora is a medicinal plant popular in the tropics where it is applied in traditional medicine practice for treatment of some illnesses but most of its acclaimed therapeutic effects have not been scientifically proven. Aim: The present study is aimed at assessing the testicular antioxidative and some biochemical effects of Eugenia uniflora in male Wistar rats. Methodology: A total of 30 male Wistar rats were divided into 5 groups of 6 animals each. Group I which served as the negative control received distilled water while group II (positive control) received 5 mg/kg Lead. Group III received 200 mg/kg BW of the extract and 5 mg/kg Lead, while Group IV received 400 mg/kg BW of the extract and 5 mg/kg Lead and the animals in Group V received 800 mg/kg BW of the extract and 5 mg/kg Lead. Administration was by oral gavage. At the end, the testes were harvested for analysis of testicular parameters. Result: The result showed a decrease in the level of Malondialdehyde (MDA) while Glutathione reductase (GSH) and Superoxide Dismutase (SOD) activities increased in some groups of animals treated with the extract and lead. The testicular total cholesterol level was significantly (P<0.05) decreased while the testicular protein level was not significantly affected in animals treated with the extract and lead when compared to the lead only group.