Background: The liver is the largest gland that plays a role in the plant metabolism and the removal of toxins from the body. Achillea millefolium is familiar herb in traditional medicine. Objective: to investigation the effect of achillea millefolium extract in experiment rats those exposed to hepatic damage by carbon tetrachloride. Methods: Achillea millefolium was collected in the province of Ibb, Yemen, and extracted by maceration method, then physical and phytochemical evaluation were performed for the extract. Finally, evaluation the effect of extract on liver function tests were done on twenty-one female rats were divided into seventh groups, two extract doses 100 mg/kg and 200 mg/kg were used, CCl4 was used for inducer liver toxicity, and liv52 drug used as positive control. Results: the extract has worthy hepatoprotective activity against CCl4 and the groups treated only Achillea millefolium extract as 100mg and 200mg the histological sections no showed any histopathological changes exaggerated from the normal in liver. Also, GPT, GOT, and ALP levels increased with CCl4 treatment but these liver enzyme levels were reduced when treated the rats by concentration 200mg/kg of extract more than by concentration 100mg/kg. Conclusion: the extract of Achillea millefolium has good hepatoprotective activity, may due to presence of flavonoids.