The study aimed at evaluating the alpha-glucosidase inhibitory potential of extracts of endophytic bacteria isolated from Ludwigia octovalvis (Jacq.) P. H. Raven (Onagraceae). Isolation of endophytic bacteria was done on supplemented and non-supplemented nutrient agar. The extracts of these endophytes were obtained after fermentation in Mueller-Hinton Broth (MHB). The inhibitory effect on the alpha-glucosidase enzyme of the extracts of endophytic bacteria was determined in the presence of starch and sucrose at 6 mg/mL at 37°C and by measuring the absorbance at 517 nm. Nineteen endophytic bacteria were isolated from the leaves, stems, roots, flowers, fruits and twigs of L. octovalvis. The extracts obtained from these endophytic bacteria all showed an alpha-glucosidase inhibitory effect. The S4155 extract showed less than 50% enzyme inhibitory activity with an IC50 of 163.98 μg/mL. Endophyte bacteria associated with L. octovalvis provided a source of bioactive compounds that can prevent or reduce the prevalence of diabetes.