Anxiety disorders are serious medical illnesses that affect approximately 19 million American adults. To cope with anxiety, people employ potentially harmful strategies like alcohol, cigarettes, medication, withdrawal etc. which may cause panic disorder to worsen. Gongronema latifolium (GL) is one of the medicinal plants used in the treatment of ailment including mental disorders. This study was therefore aimed to elucidate effects of administration of ethanolic leaves extract of fear and anxiety using light/dark transition box. Thirty (30) adult male Swiss white mice were assigned into three groups of ten mice each, thus: control, low and high dose groups placed on 0.9% normal saline, 200 mg/kg and 400 mg/kg of ethanolic leaves extract of GL respectively. All the animals were allowed food and water ad libitum. Results showed significant (p<0.001) increase in the frequency of line crosses, rearing activities in the extract treated groups when compared to the control. There was a corresponding decrease (p<0.001) stretch attend posture, freezing, grooming frequency and grooming duration in the test groups when compared to the control. These indices show that the extract treated groups of mice exhibited decreased fear and anxiety behaviours when compared to the control group. In conclusion, extract of Gongronema latifolium could be used as an anxiolytic for anxiety related disorders due to its ability to attenuate fear and anxiety related behaviours in mice.