Background: Medical students are constantly exposed to stress which when present in a small amount is beneficial as it brings out the potential of the student; but a lot of stress is injurious to the well being of the student as it exerts a negative effect on the mental health, physical health and academics of the student. Objective: To study stress, anxiety, and depression levels perceived by the 1st year M.B.B.S students. Materials and Methods: A cross-sectional study was done at Andhra Medical College from November 2020 to December 2020. This Study included 1st year M.B.B.S Students who satisfied the inclusion and exclusion criteria and were willing to give consent. Information regarding Age, Gender, Place of residence (hostel/day scholar), Motivation to take M.B.B.S course (Self-interest / Family pressure), Socioeconomic status using Kuppuswamy’s socioeconomic status scale were collected. DASS -42 questionnaire was given to the students which was used to assess the stress, anxiety, and depression levels. The data was entered into Excel 2016 and SPSS version 21 was used for analysis. Independent t-test and ANOVA tests were used to find the association between socio-demographic characteristics and DASS scores. Results: A total of 108 1st year M.B.B.S students participated in the study, belonging to the age group 18 – 20 years. The percentage of females was 55.6%. 95.4% of the students took the course with self–interest. 57.4% of students were residents of the hostel. The mean stress, anxiety and depression score was found to be 12.45 ± 5.92, 4.98 ± 2.81, 5.31 ± 3.42 respectively in this study. In this study, 49% of students were found to be in the mild stress category. Conclusion: This study showed a high prevalence of stress and a considerable amount of anxiety and depression among 1st year M.B.BS students. A student help desk comprising of a psychologist, psychiatrist, parent representative and a senior faculty member can be formed who will help the students in addressing their negative emotional states.