Background and Objective: Hepatitis C virus infection is a major cause of chronic liver disease and cirrhosis. The infection is transmitted by exposure to blood or body fluids. Since dental healthcare professionals have numerous patients and are exposed to blood, they are likely to have the maximum risk. The present study was conducted to assess the level of awareness and knowledge of Hepatitis C among third year and fourth (final) year dental students of various dental colleges in Bangalore city, Karnataka, India. Materials and Methods: A descriptive cross-sectional study was conducted among 400 students studying in third year and fourth (final) year in various dental colleges of Bangalore city. A close-ended structured questionnaire was administered which contained 11 questions. The responses were analyzed to evaluate the level of awareness regarding Hepatitis C, its mode of transmission, steps to prevent infection, associated complications and status of availability of vaccine against HCV. Results: The results of our study revealed that most of the students (72.5%) were aware of Hepatitis C vaccine but have less knowledge in terms of its mode of spread of infection, steps to prevent spread of Hepatitis C infection, associated complications and status of availability of vaccine against Hepatitis C virus. Conclusion: The present study shows that the students had poor level of awareness regarding Hepatitis C indicating need for education about HCV infection among dental students.