Background: Dengue is a tropical disease caused by dengue virus (DENV). It is one of the most significant arthropod-borne viral infections. Objectives: The aim of the current study was to characterize epidemiological, laboratory markers of dengue infection during the recent epidemic in Lahore. Methods: During the prospective laboratory based observational study, 1,082dengue suspected cases were haematological examined. CBC tests were conducted for the confirmation of dengue infection due to decrease rate of platelets count. Data regarding the laboratory features, platelet count tests were also recorded for the dengue positive patients. Results: Out of total samples 300 cases including were223 (74.3%) were male and 77 (25.6%) were female detected positive for the dengue infection. In the dengue positive patients, the highest prevalence was observed in the age group41-50 years, 76(25.3%) followed age group11-20years, 28(9.3%), 31-40 years, 37 (12.3%), 51-60 years 34(11.3%), 61-70 years, 29(9.6%), 71-80 years, 25 (8.3%), 0-10 years are 9(3%). The highly affected area in District Lahore was Johar Town block B 61(20.3%) followed by Multan chungi (17%), Ewan town and Johar town block A are 34 (11.3%), Mustafa Town 29(9.6%), Gulberg 27 (9%), Shadman 24(8%), Johar Town block D 21(7%) and Iqbal town 19(6.3%). Conclusion: The highly affected area in district Lahore was found Johar town block B. Therefore, the health department should take actions by educating the public about basic cleanliness of the environment. The community should be encouraged to participate in the control of such vector based diseases/infections.