Purpose: To assess the knowledge regarding eye donation among students of Medical, Nursing and Allied Health Sciences and gauge their willingness for eye donation. Methods: The data was collected by online questionnaire method (Google forms) from medical, nursing and allied health care students for a group of 723 students. The questionnaire comprised of 30 questions covering various aspects. Knowledge, values, attitude and spiritual believes of participants were assessed to evaluate their willingness for eye donation. Results: Among 723 medical, nursing and allied health care students 449 (62.3%) were in the age group of 21-30 years with 479 (66.4%) being females and 244 (33.8%) males. Around 80% had knowledge about eye donation and 79.1% pledged to donate eyes. There was little knowledge regarding pediatric cornea donation and transplantation. Conclusion: The awareness regarding eye donation was high but pledge to donate eye and motivate others required further motivation. Various strategies can be adopted like educating students from all walks of life, focusing on strengthening the link between the hospitals and the rural population can further enhance the corneal procurement rate.