The aim of the present study was to evaluate the antibacterial activity of Moringa oleifera leaf extracts, Escherichia coli and Klebsiella were used. The bioactive compounds Extracted from M. oleifera leaves by using many solvents, ethanol, ethyl acetate, water and methanol. The qualitative phytochemical analysis of M. oleifera leaves were carried out using standard procedures to identify the constituents of bioactive compounds (Alkaloids, Flavonoids, Tannins and Phenols). The methanol crude leaves extract were used to determine the Minimum Inhibitory Concentration on E.coli and Klebsiella by using the method of Greenwood as well as the inhibitory zone. Results; this study shown that the extract is active against bacterial isolates, whereas the inhibitory effect of the isolate is dose depending, where higher activity was clear by dose 200 mg/L. Also, the sensitivity of the bacterial isolate to the extract differs whereas Klebsiella is more sensitive to the extract with average zone 3.73 mm while E. coli less sensitive by average zone of inhibition 3.47 mm at a maximum concentration 200mg/Lin comparison with a control.