Radish is consumed and cultivated globally and is reflected as a part of human-diet, although this root vegetable is not common in many populations. Different varieties of radish have the different soil and climate requirements for better growth and yield but the most beneficial and important agro-technique is nutrition for better performance. For better quality and optimal radish production, fertilization by organic, inorganic and bio-fertilizers are very important constituents. Other pharmaceutical uses of radish include treatment of chronic diarrhea, neuralgic-headache and sleeplessness. Toxicity of Ni in radish plant is confirmed by inhibition of growth, chlorosis, necrosis and wilting. This report based study conducted to investigate the biological, physiological effects of heavy metals on the radish. In this study it was noticed that nickle sulphate act as suppressor on growth of the plant and it also decreased the content of carotenoids and chlorophyll contents such as Chlorophyll-a, b. Statistical analysis was achieved after collection of data by using appropriate computer software such as ANOVA.