Saudi Journal of Medical and Pharmaceutical Sciences (SJMPS)
Volume-7 | Issue-06 | 270-274
Review Article
“Biochemical Features, Genetic Breeding Approach, Salient Features and Plant Molecular Approach to Target Plant Genes”
Muhammad Riaz, Muhammad Ehsan Haider, Arbaz Khan, Muhammad Husnain Abbas, Muhammad Afzal, Saifullah, Sara Siddique, Muhammad Haseeb Anwar Tarar
Published : June 20, 2021
Abstract
Different methods are used for the introduction of foreign DNA in the genome of a plant. These processes includes the biological methods which are based on pathogenic bacteria A. rhizogenes and A. fumefaciens, or the chemical and physical coordination’s like microprojectile-bombardment, micro-injection, chemical poration and electroporation. The bacterial transformation is the straight gene transferring mechanism through which the some of the bacteria intake the foreign DNA from environment. The protoplasts of plant treated with the polyethylene glycol take up the DNA more rapidly from the surrounding, and this DNA can be integrated stably in to plant chromosomal DNA. Biolistics contains acceleration of higher mass transporter elements (commonly made up of tungsten, gold or platinum) roofed with the genes which passed through cells, separating the DNA inside by the adsorption method. SiC whiskers are like a needle having a size of 20μm in length. These whiskers helps in penetrating the plasma membrane and cell wall of targeted cell for transferring the wanted DNA and hence, the transformants are attained.