False creatinine results estimated by conventional Jaffe’s endpoints are still a problem in the laboratories. We hypothesized that delay in absorbance measurement may influence the concentration of creatinine measured by Jaffe’s conventional method. This study compared the agreements between creatinine measured by conventional Jaffe’s endpoints and kinetic Jaffe’s methods and the influence of delayed absorbance reading on creatinine concentrations in kidney disease patients. A cross sectional study conducted in one hundred (70 males and 30 females) adults with kidney dysfunction. Serum creatinine was measured by Jaffe endpoint method and by Jaffe’s Kinetic method. Significant increase was observed in the creatinine measured by Jaffe’s endpoint method compared with Kinetic method. The serum creatinine concentration measured by Jaffe’s endpoint methods increases with increase time. This study has indicated that conventional Jaffe endpoint and kinetic methods show poor agreement for the estimation of serum creatinine in the presence of renal dysfunction, the kinetic method performed slightly better. This study also suggests that delay in absorbance reading for Jaffe endpoint reaction is a factor which influences serum creatinine concentrations.