Despite the advent of generic drugs, many treatments still remain financially inaccessible to economically disadvantaged populations who constantly continue to turn to bioactive molecules from nature to find essential remedies that are gentle, more effective and with few side effects. Medicinal plants are still the primary reservoir of new drugs. The material used during this study consisted of plant material, animal material and technical material. 500 g of the sprayed plant drug was macerated in 2.5 L of distilled water for one duration of 48 hours. Numerous secondary metabolites of the aqueous extract of the leaves of Diospyros hoyleana F. White have been demonstrated. The acute oral toxicity experiment was conducted according to OECD protocol guideline 423. The mass of crude extract obtained after drying in an oven at 60 °C. was 64 g with a yield of 12.8%. Tests for Flavonoids, Saponins, Phenols, Tannins and Anthraquinones were found to be positive with the aqueous extract of the leaves of Diospyros hoyleana F. White while those for Alkaloids, Sterols, Terpenes and Coumarins were found to be Negative. After the acute oral toxicity study, no abnormalities of the physiological parameters observed in rats were observed. In addition, no deaths have been recorded. The LD50 was therefore greater than 5000 mg/kg. The body masses of the animals were generally increasing during the study regardless of the chosen batch. The rats were sacrificed and the masses of the internal organs were weighed. Four blood biochemical parameters of the groups of rats studied were assayed.