Transseptal access from right atrium to left atrium through the fossa ovalis using transseptal puncture and patent fossa ovalis repair are widely used cardiac techniques. These techniques require through knowledge of the cardiac anatomy especially of the fossa ovalis .Evaluation of the various morphological parameters of the fossa ovalis forms an important prerequisite before undertaking any surgical procedure in this region. This study was conducted on 60 cadaveric hearts from the department of Anatomy, Government medical college, Nagpur. The right atrium was opened and the fossa ovalis was studied for its shape, size, floor and the prominence and extent of the limbus fossa ovalis was noted. The fossa ovalis was observed for the presence of any patent foramen ovale or probe patency. In majority of the cases the shape of fossa ovalis was found to be oval 66.7% cases, the average craniocaudal diameter was found to be 15.03mm, and average anteroposterior diameter was found to be 14.44mm. Limbus fossa ovalis was found to be raised in 83.33% cases and flat in 16.66% cases. Probe patency of foramen ovale was found in 15% cases but no case of patent foramen ovale was found. The floor of fossa ovalis was found to be flat in 65% cases and aneurysmal in 35% cases. In 10% cases membranous structure with multiple fenestrations or fine strand like structures were seen attached to the lower part of fossa ovalis. The findings of the present work are of immense value to interventionists and cardiologists