Aim: To evaluate the efficacy of amniotic membrane transplantation in corneal ulcers and to observe the complications of amniotic membrane transplantation in corneal ulcers. Material and methods: Ours was a prospective cross- sectional study which was conducted over a period of 1 year at Regional Institute of Ophthalmology, PGIMS, Rohtak on 30 patients of microbial keratitis on whom amniotic membrane transplantation was done. Signs of ulcer healing- pain, congestion, size of ulcer, BCVA, anterior chamber depth and anterior chamber reaction were recorded on every subsequent follow up. Result: There was significant reduction in post-operative pain and congestion. Anterior chamber depth was not changed significantly. Conclusion: Amniotic membrane transplantation can be used as an adjuvant treatment in bacterial and fungal corneal ulcers for early healing, less vascularisation and scarring and better final visual outcome.