In mammals, hair cycle occurs continuously in three cycles: anagen, catagen, telogen. One of the fibroblast growth factor (FGF) family, FGF5, have been known to act as an inhibitor of hair growth in the transition from anagen to catagen. The FGF5 gene is composed of a long form (FGF5) and a short form (FGF5s). We examined the effect of FGF5 isoform on hair growth in the cultured keratinocytes. To analyze the effect of FGF5 isoform the keratinocytes, we overexpressed FGF5 isoform and gene expression change was observed with RT-PCR. Expression of hepatic growth factor (HGF) gene was increased when FGF5s was overexpressed by adenovirus (Ad) in keratinocytes. BMP6 tend to be decreased in the group transfected with Ad/FGF5 virus than in the control group. Taken together, FGF5s might activate HGF in hair growth and FGF5 may inhibit BMP6 expression in hair growth suppression.