Shark liver oil is one of the sources of cellular energy and provides repair and restoration of damaged cells, and supports in protection of the body from the emergence of tumorous conditions. The Shark liver oil was tested orally at the dose of 1gm/kg/oral body weight, on gastric ulceration experimentally induced by Ethanol. The parameters considered to assess the Mucoprotective activity were percentage of ulcer, ulcer index, percentage of inhibition, pH, free acidity and total acidity, GSH, LPO and ALP. Pre treatment with the Shark liver oil resulted in significant decrease of the ulcerated area and reduced pH, free acidity and total acidity.