The existence of National Forensic DNA Database enormously helps investigations & control of crime. But criticisms have been raised at the ethical & social risks from misuse of DNA database. In India, there is no legislation on DNA profiling. Hence, peoples’ support is required. So, we aimed to study the knowledge & attitude about the benefits & risks of National Forensic DNA Database among 400 medical students. We found that the knowledge about the use of DNA fingerprinting as an evidence is good. The knowledge about National Forensic DNA Database & its current status in India is poor. Most of them (87.5%) opinioned that it’s a step forward in criminal investigations. 42% felt it’s beneficial. Many of them doubted its efficiency (40%), safe guarding (53%) & its establishment (47.5%) in India. Many of them feel that they have privacy issues (62.7%) & it has potential to harm (39.3%). 51.5% felt it should be a permanent record. Most of them supported for inclusion of all crime scene stains, suspects, recordable offence, missing persons & unidentified dead bodies DNA should be profiled. 42.75% were willing to provide their DNA samples. Hence, we found that the knowledge of medical students about National Forensic DNA Database is not adequate. This will lead to lack of support & may be interpreted as violation of human rights. But most of them supported the need for such a database; hence creating awareness on our part will definitely help in improving the people’s understanding.