Background: The prevalence of hypothyroidism in the reproductive age group is found up to 4%. In many studies it had been claimed that, thyroid status is directly involved in women reproductive system. Although it differs in several places, communities, geographic areas and age-groups but it deserves more attention from the reproductive health physicians and even from world communities. Hypothyroidism can be easily detected by assessing serum thyroid stimulating hormone levels. Aim of the study: The aim of this study was to dig out the prevalence of hypothyroidism in infertile women and to assess their responses in treatment procedures. Methods and Materials: This was a descriptive observational study conducted in Cumilla Medical College and Hospital, Cumilla, Bangladesh, during the period from January 2019 to December 2019. In total 128 women attended the infertility Unit of the mentioned hospital for fertility treatment were finalized as the study population of this study. The regular investigations CBC, blood sugar of 2 hours GTT, TSH and Prolactin was done. Hypothyroidism was diagnosed when increased TSH and normal FT4. Levothyroxin were given hypothyroid patients depending upon TSH levels. Results: Total 128 participants 56.25% (n=72) were with primary infertility and the rest 43.75 %(n=56) were with secondary infertility. In total 23% (n=29) participants were hypothyroid whereas the rest 77% (n=99) participants were euthyroid and/or hyperthyroid. Among the hypothyroid (n=29) participants, 20 were subclinical hypothyroid which was 69% among hypothyroid participants and 15.63% among the total participants. In total 9 were frank hypothyroid which was 31% among hypothyroid participants and 7.03% among the total participants. We found, the mean (±SD) S. TSH, S. FT4 and S. Prolactin were 7.23±2.45 mIU/L, 16.95±3.32 pmol/l and 51.83 ±10.70 ng/ml respectively. Conclusion: According to the findings of this study we can conclude that, careful diagnosis and treatment of hypothyroidism can ensure benefit a lot rather than going for unnecessary hormone assays and costly invasive procedures. The findings of this study may be helpful in the treatment arena of infertility of women and in further similar study.