Background: Presence of septum and anatomical knowledge of hepatopancreatic ampulla morphometry plays major role in understanding safety surgical procedures of duodenal cancers Objective: to study the length of hepatopancreatic ampulla in Karnataka population. Materials and Methods: 50 formalin fixed cadavers (Male - 41; Female - 9) with age group ranged between 35 - 60 years from the departments of Anatomy, J.J.M Medical College, Davengere, Karnataka & Government Medical College, Palakkad, Kerala to study the length of hepatopancreatic ampulla and its clinical importance. Coloured solution was injected at the junction of major and minor pancreatic duct to study the length of hepatopacreatic ampulla. The length of the hepatopancreatic ampulla was measured till its opening into duodenal papilla. Results: The presence of hepatopancreatic ampulla was observed in 39 specimens out of 50 cases. The measurement of length of the hepatopancreatic ampulla revealed that the length varied from 2 mm to 10 mm. The length varied from 1 to 2 mm in 3 (6%) out of 50 specimens and 3 mm or more in 36(72%) specimens. The hepatopancreatic ampulla was absent in 11 specimens (25%) due to presence of complete septum till the duodenal orifice in the present study. Conclusion: The present study gives basic knowledge in understanding the extension of hepatopancreatic ampulla from its point of origin to termination before planning a surgery.