Background: Exposure to environmental heavy metals predispose humans to health risks and hazards. Increased risks of chronic diseases have been attributed to increased exposure to heavy metals. Studies have linked early onset of chronic diseases in HIV-infected persons to exposure to heavy metals in the environment. Given the massive industrialization and crude oil exploration in the Niger Delta, there is huge environmental pollution in the area. Objectives: The study sought to evaluate the presence of blood lead, cadmium and vanadium (notable heavy metals associated with crude oil exploration) levels of HIV-positive and HIV-negative volunteers in Port Harcourt, Niger Delta Nigeria. Materials and Methods: The study evaluated the presence of lead, cadmium and vanadium in HIV-positive and HIV-negative volunteers in Port Harcourt, Nigeria. Blood samples of a total of 100 subjects (60 HIV-positive and 40 HIV-negative subjects) with age range 15->60 years were analysed using Solar Thermo Elemental Flame Absorption Spectrometer (S4 710). Results: Mean blood levels of lead, Cadmium and Vanadium obtained were 2.00±6.25; 1.70±11.20 and 0.03± 0.04 ppm respectively for HIV-subjects and 0.15±0.28, 0. 04±0.05, 0.01±0.02 ppm for HIV-negative subjects. Conclusions: This study demonstrated that HIV-positive subjects had significantly higher levels of all the heavy metals compared to HIV-negative persons thereby predisposing them to be more at risks of the metals.