Standardization plays a significant part in the production of phytopharmaceutical of standard quality as the quality standards are based on proper selection of raw materials. Celastrus paniculatus Willd (Celastraceae) is the conventional Ayurvedic medicinal plant used for centuries as a memory enhancing, anti-inflammatory, analgesic, sedative and antiepileptic agent. The decoction of seeds is given in rheumatism, gout, paralysis and leprosy. High Performance Thin Layer Chromatography (HPTLC) technique is a sophisticated and automated form of the thin-layer chromatography (TLC) with enhanced and advanced separation efficiency and detection limits and is often an outstanding alternative to GC and HPLC. Applications of HPTLC include phytochemical and biomedical analysis, herbal drug quantification, active ingredient quantification, fingerprinting of formulations, and check for adulterants in the formulations. Present investigation includes examination of morphological and microscopic characters, ash value, extractive values and phytochemical evaluations including qualitative chemical examination of active constituents. The Pet. Ether extract (i.e. oil) contains different fatty acids and they were converted into their methyl esters for their assessment by HPTLC.