Background: Hydroxychloroquine Sulphate, is an antimalarial drug used in treatment and prophylaxis of malaria, and other conditions like rheumatoid arthritis, and tried as a prophylaxis of venous thromboembolism. It is one of the important choices in COVID-19 management, as shown by about 80% improvement in pneumonia, and prevention of further disease progression to severe conditions upon following Hydroxychloroquine therapeutic regimen. Aim: The aim of this work was to develop an economical, precise, accurate and specific analytical method for quantitative estimation of Hydroxychloroquine sulphate in pharmaceutical product for the purpose of using it as a quality control tool for testing Hydroxychloroquine sulphate products pre-market and post-market distribution. Insuring the presence of labelled drug amount in the dosage from. Methods: Determination of HQC in commercial pharmaceutical formulations dispensed in hospitals and community pharmacies and administered by patients, by in house development of a validated, selective and sensitive High Performance Liquid Chromatography (HPLC) test method, where, the procedure used is in accordance with published literature data, pharmacopeia, and international guidelines. Results: The method is specific and selective, and showed linearity R2>0.999 within concentration range of 25-300 µg/mL, accuracy results within the range of 98% - 102%, precision CV% less than 2%. The assayed tablets mean recovery are 99.045%. Moreover, dissolution results are fulfilling the required limit of 70% percent dissolution within 60 minutes. Conclusion: The in-house developed analytical method is easy, and cost effective for use in quantification of HQC.