Abstract: Equality between men and women is a fundamental right and a common policy of many governments in the world today. Gender mainstreaming is the process whereby gender concerns are raised routinely within everyday operations of an institution or organization and resolved in a gender manner in normal operations. The well-being and the equality between men and women are addressed by gender mainstreaming. The purpose of this study was to establish the effectiveness of gender mainstreaming initiatives employed in enhancing gender equity in the Ministry of Agriculture, Livestock and Fisheries in Kirinyaga County, Kenya. The specific objectives of the study were to establish how recruitment and selection, employee awareness, a gender office and implementation of gender mainstreaming policy affect gender equity in Ministry of Agriculture, Livestock and Fisheries in Kirinyaga County. The study employed a descriptive survey research design and targeted all fifty (50) Ministry employees. Descriptive data analysis was done which generated mean and standard deviation with the help of Statistical Package for Social Sciences (SPSS). Frequencies and percentages were also generated. The study established that there was a close relationship in terms of proportions of the respondents between a yes (54%) and no (46%) responses on training.The study established that the Ministry ensured fair recruitment and selection based on Gender Equity to a great extent (36%).It is therefore recommended that the Ministry through their Cabinet Secretary provides statistics of the incoming employees based on gender. The researcher also recommends that the Ministry clearly outlines what constitutes gender mainstreaming training. The study also found out that the Ministry of Agriculture, Livestock and Fisheries had neither done much (40%) on sponsoring gender balance awareness training nor done much(34%) on implementing gender balance awareness training. The researcher therefore recommends that awareness training should be enhanced.