Crops are very important in order to accommodate world population for food as well as industrial purposes. MiRNA (micro-RNA) have been considered as an important and crucial factor for manipulation of crops to make them more productive and resistant against harsh environmental stressed conditions. Now, these non-coding special sequences have been used successfully for gene-expression and regulation such as gene integration, slicing, signal transduction, pre and post translational modification, boost up metabolic pathways, enhancement of crop growth and developments and much more traits which is significant contribution in genetic engineering technologies for crop modifications. Genomic expression factors have been modulated through unique-miRNAs sequence which takes up toward the next generation specific targets that would have been adapted under biotechnological mechanisms and then these technologies could be used for improving the agronomic traits of various crops further down collection of high productive results. Significant strategies have pointed out to overcome the drawbacks during crop-manipulation. In this review, work for diversified and recently identified sequences of micro-RNA is studied; and production of valuable crops in order to have better agronomic properties to fulfill the requirements of food and industry with significant sustainability under stressed conditions. Genetic regulation as well as expression through miRNAs in genetic engineering will facilitate better for crop modification with quality and would be considered as positive step toward improving the economy of country.