This study aims to analyze the effectiveness of temephos against Aedes aegypti larvae in the Buffer Airport and Poso Sea Port area. This type of research is quasi-experimental. The research sample used the first-generation instar Fed 1 Aedes aegypti larvae obtained from mosquito breeding. A total sample of 240 tails consisted of treatment and control with 3 replications each. Testing uses 1% temephos concentration. The statistical test used in univariate and probit analysis. Based on the results of the study showed that the Poso Airport Buffer, Aedes aegypti test larvae were susceptible to temephos 1% with a total mortality of 100% of the test larvae. where the WHO standard criteria state for larvae of the test 98-100% Vulnerable, 80-98% Tolerant deaths, and <80% resistant. Likewise, with the Poso Port Buffer region, the Aedes aegypti test larvae are susceptible to temephos 1% with a total of 100% average adult mosquito mortality. the overall lethal time values (LT50, LT90, LT95, LT99) in the Poso Sea Port Buffer region are higher than in the Poso Airport Buffer area, where the Poso Sea Harbor Buffer area for 1% temephos requires a longer time to kill the Aedes aegypti test larvae, compared to the Poso Sea Port Buffer region. by poso airport. Especially to kill 99% of test larvae in the Poso Sea Port Buffer region, temephos 1% takes 147.2 minutes or 2 hours 27 minutes while for the Poso Airport Buffer region it only takes 119.86 minutes or 1 hour 59 minutes to kill 99% of larvae test.