The study examined the prevalence of HIV among pregnant women in three selected hospitals in Port Harcourt namely; military hospital, St. Margaret maternity home and El-Joe dam specialist hospital. This study was aimed at determining the Prevalence of HIV/AIDS among pregnant women in Port Harcourt, Nigeria. The population of the study was all the pregnant women that registered for antenatal at the three selected hospitals. The sample size was 400 randomly selected. 300 pregnant women were sampled at Military hospital, 50 each at St. Margaret maternity home and EL-Joe dam specialist hospital. The “prevalence of HIV /AIDS a close-ended questionnaire” used to collect data. The results were tabulated. The blood sample for the test was collected by vein puncture into EDTA. The confirmatory test was done using ELISA, PCR and western blot Methods. A relatively high number of seropositive women fell in the age brackets of 30-34 which is 42.11% years while the least prevalence was between the ages of 20-24 years. Out of the four hundred (400) subjects screened thirty–eight seropositve which can be translated to a prevalence of 9.5% in Port Harcourt. The research work indicates that there is high HIV prevalence among pregnant women more especially during their sexual active age. The finding of this work is not at variance with work done in other parts of the world, based on the literature review. The research work indicates that there is HIV prevalence among pregnant women.