In this study, changes in some enzymatic activities in different tuber parts of "Florido" and "Bètè-bètè" cultivars belonging to Dioscorea alata species were investigated during post-harvest storage. Indeed, the studied enzymatic activities were those of acid phosphatase and pNP-glycosidases (α-glucosidase, α-fucosidase, α- galactosidase, β-glucosidase and β-galactosidase, α-mannosidase) and polysaccharidases (amylase, cellulase and inulinase). The results showed that the different tuber parts of both cultivars contained acid phosphatase and α-mannosidase activities. Besides, "Bètè-bètè" cultivar also possessed α-galactosidase. As for the polysaccharidases activities, the different parts of both cultivars had amylase and cellulase activities. Otherwise, the detected acid phosphatase, α-mannosidase and α- galactosidase activities in yam tuber part decreased with the storage time while the amylase and cellulase activities increased with the storage time. The enzymatic activities were found to be high in the distal tuber part for both cultivars. This work revealed that both cultivars didn’t contain α-glucosidase, α-fucosidase, α-galactosidase, β-glucosidase, β-galactosidase and inulinase activities. Our results could constitute an attractive biochemistry in the understanding of yams physiology during post-harvest storage.