Agarwood oil is a highly priced type of oil due to its unique aroma. Agarwood oil is widely used in perfume, medicine and cosmetic industries due to its unique properties and high medicinal value. The oil is generally extracted from the fragrant resins that form in the heartwood of agarwood tree. These unique properties of agar oils are contributed by the presence of certain chemical compounds. The market demand for agarwood oil is very high. Agarwood oil is traded based on grade, corresponding to expensive and cheap price. Currently, the grading of agar oil depends on its physical appearance such as color, odor and essential oil components, like present of sesquiterpenes. The present research analyzed essential oils from Aquilaria malaccensis obtained from Moulvibazar, Bangladesh based on GC-MS data and antimicrobial properties. GC-MS analysis found Sesquiterpenes, alcoholics, fatty acids and other chemical groups in the exportable agar oils. Farm-1 contained 94.95%, Fram-2 contained 95.37% and Farm-3 contained 93.90% sesquiterpenes which is the most important properties for stronger odors and high quality. All the agar oils contained about 75% 5-Azulenemethanol-1,2,3,4,5,6,7,8-octahydryo.alpha.alpha.3.8- tetramethyl-3,7-Cyclodecad iene-1- ethanol.alpha.alpha.4,8–tetramethyl-[s-(Z,Z)]. They also contained other sesquiterpenes like Ledene oxide- II; Aromandendrene; Bicyclo[4.4.0]dec-5-ene,1,5-dimethyl-3-hydroxy-8-(1-methylene-2-hydroxyethyl-1)- 1H-3 a,7-methanolazulene, deca hydro-1,4,9,9-tetramethyl-Diethyl Phthalate; Isoaromadendrene epoxide. Other groups like alkane ~2%, alcoholics ~1% and fatty acid were found above 2%. The essential oils from all the farms except farm-2 have shown non-significant sensibility in antimicrobial test against E. coli, S. aureus, Salmonella,Vibrio.