There is the need to move away from chemical growth promoters to natural products in the poultry industry, as these have negative effects especially with regard to fat accumulation. In this regard, Moringa oleifera is known to have a number of beneficial effects on hematology and serum chemistry of humans. This study was therefore conducted to investigate the effects of M. oleifera leaves on lipid profile and some serum enzymes in Nigerian local Chickens. Thirty adult Chickens were used for the study. The birds were randomly assigned to three groups(n=10).One group served as control while two treatments received a daily oral dose of 12.5mg and 25mg Moringa leaf solution respectively. The experiment lasted for 28 days. Data obtained were subjected to analysis of variance. Results indicate that the mean values of total cholesterol, high density lipoprotein cholesterol, low density lipoprotein cholesterol, Triglycerides were statistically similar for all groups. Furthermore, mean values for serum levels of aspertate aminotransferase, alanins aminotranferae and alkaline phosphatase were statistically similar for all treatment groups. It is concluded that the administration of M. oleifera leaves at the dose used in this study has no significant effects on lipid profile and the serum enzymes evaluated. It is recommended M. oleifera leaf meal can be used to supplement expensive protein sources in chicken diets.