Present study was designed to evaluate the ameliorative effect of Tribulus terrestris (TT) and vitamin C against permethrin induced oxidative stress in goat testicular tissue in vitro. Testicular tissue was divided into control and three experimental groups EI, EII and EIII. All the groups were cultured in TCM-199 nutritive media at 95% humidity, 5% and 39℃ in CO2 incubator for 4hr. and 8hr. duration. In group EI, the testicular tissue was exposed to 100µg/ml and 200µg/ml of permethrin. EII group was exposed to 100µg/ml and 200µg/ml of permethrin along with 0.1mmol of vitamin C. While EIII group was tested for 100µg/ml and 200µg/ml of permethrin along with 100µg/ml hydro-alcoholic extract of TT. Present study revealed that the activity of antioxidant markers such as catalase (CAT), glutathione peroxidase (GPx) and reduced glutathione (GSH) were significantly (p<0.05) decreased in permethrin treated groups as compared to control in both dose and time dependent manner. Maximum decline (62.69%, 39.4%, 51.5% decrease in level of CAT, GPx, GSH respectively) in the antioxidant status of the tissue was reported at 200µg/ml for the duration of 8hr. However, 100µg/ml of permethrin resulted in 29.3%, 14.3%, 29.5% decrease in CAT, GPx, and GSH level respectively for the same exposure duration i.e. 8hr. The activity of antioxidants was found to be improved in vitamin C (EII) and TT (EIII) supplemented groups (p<0.05). From the present study, it is concluded that permethrin induce a decline in the status of antioxidant enzymes. Vitamin C and TT are potent antioxidants that mitigate the toxic effects of permethrin by elevating the level of antioxidants.