Urolithiasis is the process of forming stones in the kidney, bladder, and urethra. The prevalence of urolithiasis is approximately 2 to 3 percent in the general population. The development of the stones is related to decreased urine volume or increased excretion of stone-forming components such as calcium, xanthine, oxalate, cystine, urate and phosphate. Herbal plants have been the basis for medical treatments since ancient time, and such traditional medicines still widely practiced today. The scope of herbal medicine is sometimes extended to include fungal and bee products, as well as minerals, shells and certain animal parts. In this study in vitro antiurolithiatic activity of Citrus limetta was done by two methods. Method A. was test by turbidity method in which method activity of the extract was tested in terms of inhibition of calcium oxalate formation by the method of Prachi Khare et al. with modification and Method B.was test by calcium oxalate dissolution method in this second method the role of plant extract in dissolving the already formed calcium oxalate stones nucleus in artificial system. The experiment was carried out in four arrangements according to the method of Unnati Atodariya et al. with modification. In the method A it was observed that the extract with 100 mg/ml concentration has shown higher percentage of inhibition (35.29%) of calcium oxalate formation than the extract with 250 mg/ml concentration. While the standard drug has shown percentage inhibition of 58.82% and in Method B the Low Dose of the Extract (50 mg/ml) had shown 15.2% of dissolution and High Dose of the Extract (100 mg/ml) had shown 27.7%, while the standard cystone (50 mg/ml) had shown 68% of dissolution of calcium oxalate. From the study it can be concluded that the hydroethanolic extract of Citrus limetta possesses in-vitro anti urolithiatic activity which can be due to the presence of phyto-constituent such as flavonoids present in it.