The aim of this study was to evaluate the seroprevalence of toxoplasmosis infection in pregnant women in Setif region and to identify the main risk factors involved. The different parameters were collected by direct interviews and using standardized ELIFA technique for detecting the anti-toxoplasmic antibodies IgG / IgM and determining the immunological status of this sample. Our results showed a general lack of Toxoplasmosis awareness, a prevalence of 58% of seronegative pregnant women and 42% of seropositive women they may have risks of seroconversion as the case observed in our study. The statistical analysis confirmed that the consumption of undercooked meat is the main factor of contamination. This study has the advantage of setting up a serological monitoring of toxoplasmosis according to a legal framework with the aim of detecting and servile seronegative women, hence the importance of education and information in terms of prevention.