Micro algae Dunaliella salina is isolated from Sambhar Salt Lake, which has very high amount of carotenoids. We studied growth of D. salina in various concentration of EMS through rapid addition and having 1 hour treatment in dark to find out effective and efficient dose of EMS for D. salina. Studies revealed the effectiveness and efficiency decreased with increase in EMS concentration. Lower concentration of EMS was more effective and efficient for promoting growth. Maximum growth was found in control as compare to EMS treated cultures. The concentration of EMS chosen reduced the rate of growth, cell movement and cell survival as compare to control. This reduction increased with increasing concentration of EMS. Thus the mobility and survival rate of D. salina was negatively related to the concentration of EMS. Rapid addition of EMS exhibited high growth in comparison to 1hour treatment of EMS in dark.