Aqueous plant extracts in ophthalmic preparations is not uncommon. Folklore (Indigenous) herbal preparations in this regard claim to cure eye diseases need to be explored. Antimicrobial potency of such herbal formulation is studied with comparison of its efficacy against selected pathogens associated with eye diseases helps for its standardization and validation as well. Hence Herbal formulations were taken and their antimicrobial efficacy was compared with standard treatises against wide range of eye pathogens. Both agar well and disc diffusion methods were followed for this purpose. Effect of extract on viability of microbes was also studied taking absorbance data at 600nm spectral wavelength. The multidrug resistance of the strains was also tested prior to their use in antimicrobial sensitivity test. The antibacterial efficacy of standard eye drop showed highest zone of inhibition against E. coli and lowest against P. vulgaris. Similarly the indigenously prepared eye drop was found to be with fewer efficacies. However the 50% diluted ophthalmic preparation showed higher degree of inhibition was revealed. The study explored that the diluted and standardized indigenously prepared ophthalmic solution can be used as a more potential drug at least against microbe associated and induced eye diseases.