Presently an experiment was conducted so as to understand the moulting behaviour in freshwater crab Maydelliatelphusa masoniana in the laboratory conditions. Out of eight crabs only five could moult while three died during ecdysis. All the crabs exhibited the same pattern of moulting viz., appearance of double lining in pereiopods, the old carapace becoming light in colour follow by its split along the posterolateral margin of the dorsal side, anterolateral border of the ventral side and posterior border of the chelipeds and legs. A new pre-exuvial soft layer is formed below the old carapace. The whole process of moulting got completed within 20-25 minutes.