The present study was aimed to look for the effect of a natural compound i.e. Crocin on the viability of mature adipocytes and on inhibition of adipocyte differentiation. The 3T3-L1 cell line is one of the most well-characterized and reliable models for studying the conversion of pre-adipocytes into adipocytes. 3T3- L1 pre-adipocytic cell line was used and differentiated into mature adipocytes using standard adipogenic media. The MTT assay was used for the assessment of cell viability whereas effect on adipocyte differentiation was visualised by Oil Red O staining. The changes in relative lipid content were monitored spectrophotometrically at 510nm. Mature adipocytes when treated with Crocin decreased cell viability in a dose dependent manner. It also showed an enhanced inhibition of adipocyte differentiation as monitored by Oil Red O Staining. The western blot analysis revealed significant increase in the expression levels of AMP activated protein Kinase which is a key regulator of energy homeostasis. Thus, the crocin seems to affect multi-potent anti-obesity parameters and may have promising benefits for the treatment and prevention of obesity.