The experiment was conducted with 34 local rice genotypes with one check varieties at the Sher-e-Bangla Agricultural University experimental field to study characterization and genetic diversity of 35 local rice genotypes and to find out the association among the genetic characteristics. PCA showed the highest inter-cluster distance was observed between the cluster II and V (46.71) followed by III and V (46.40) and IV and V (45.43). Genotypes from these three clusters may be involved in hybridization as wide genetic variations exist among the groups. Cluster IV exhibited the highest intra-cluster distance (38.36), while the lowest distance was observed in cluster V (23.09). Further study of this experiment is needed in different locations of Bangladesh for accuracy of the results obtained from the present experiment. Considering diversity pattern and other agronomic performance lines G-10, G-13, G-15, G-18 and G-24 could be considered suitable parents for efficient hybridization in future.