Candida fermentati SA3-7, a haploid yeast strain was treated by ultraviolet (UV) radiations and Dimethyl sulphonate (DMS) for enhanced invertase (EC 3.2.1.26) production. The UV induced mutagenesis did not produce any stable mutant with improved enzyme production. The culture was subjected to DMS treatment. The mutant DMS-4 obtained after exposure with 0.06 mg/ml of DMS for 45 min gave many fold increased enzyme production (34.12 U/ml) compared to the wild. After optimization of incubation time (48 h), sucrose concentration (5 g/l), initial pH (6.0) and inoculum size (2 % v/v, 16 h old), the enzyme production reached 45.65 U/ml with a remarkable >40 fold enhancement. On the basis of kinetic parameters markedly Qp (0.872 U/g/h), Yp/s (2.403 U/g) and qp (0.209 U/g yeast cells/h), it was concluded that the mutant was a hyper producer of enzyme and has a faster growth rate. The rate of volumetric production was 25 fold improved over the parental strain. The enhancement in the enzyme production is highly significant (HS, LSD 0.045, p≤0.05).