Medicinal plants are source of a great economic value. Plants herbs are naturally gifted at the synthesis of medicinal compounds. The extraction and characterization of bioactive compounds from medicinal plants have resulted in the discovery of new drugs with high therapeutic value. Baobab Adansonia digitata L., (Malvaceae) seeds were collected from El-Obeid, North Kordofan state Sudan, and were extracted with hexane as solvent the extracted oil was analyzed by GC-MS. Twenty four constituents were detected the major constituents are: 9-octadecanoic acid methyl ester (25.08), 9,12-Octadecadienoic acid (Z,Z)-, methyl ester (23.08), Hexadecanoic acid, methyl ester (22.78), Methyl stearate (6.86) and Methyl 2-octylcyclopropene-1-heptanoate (6.08). In the well diffusion bioassay the oil showed significant activity against Bacillus subtilis, Pseudomonas aeroginosa, Staphylococcus aureus, and the yeast Candida albicans but it was partially active against Escherichia coli. The antioxidant activity of the extracted oil was evaluated using the standard 2, 2diphenyl-1-picrylhydrazyl (DPPH) 0.5 ml. The antioxidant activity of the extracted oil was 19.00 ± 0.08