The nature has provided an entire warehouse of remedies to cure ailments of mankind. About 80 per cent of the world's population depends partially or wholly on traditional medicine for its primary health care needs. Herbal medicines as the key remedy in traditional medical system have been used in medical practice for past era and have made a great involvement to maintain human health. Thus Pharmacognostical study appears to be of enormous value in identification of commercial samples of the market to find their authenticity and establishing identity of adulterant or substituent. In addition lack of standardization, dishonest commercial practice of adulterating and substituting the genuine herbal drugs are posing great obstacle in popularizing the time-tested herbal-based traditional medicines. The present study was focused on preliminary pharmacognostic and phytochemical investigations for determining and establishing the identity, purity and quality of the plants. The plant was subjected to determination of various physicochemical parameters including ash values (total ash, water soluble ash) and extractive values (alcohol soluble extractive, water soluble extractive). The powdered crude drug was extracted successively with various solvents with increasing polarity and further the extracts were subjected to phytochemical screening for the identification of various phytoconstituents. The study of heavy metal analysis, total bacterial count & test for Aflatoxins studies was also carried out as WHO guidelines. More over the fingerprint profile of Desmostachya bipinnata was established using thin layer chromatography(TLC) methods. The results of the TLC profiling of the extract confirm about the presence of various phytochemicals.