Introduction: Chronic kidney disease (CKD) is one of the most common non communicable diseases throughout the world. According to the data of Bangladesh Renal Registry almost twenty millions of Bangladeshi adults are suffering from various stages of CKD. Many of these CKD patients ultimately developed ESRD when life is not sustainable unless renal replacement therapy is initiated. Objective: The purpose of the present study was to estimate the rates of infections and clinical and microbiological evaluation of bloodstream infections in patients undergoing maintenance hemodialysis. Methods: This cross sectional study was conducted from January 2014 to December 2015 for a period of 2(two) years in the Department of Nephrology at Bangabandhu Sheikh Mujib Medical University, Dhaka. All adult patients underwent hemodialysis of both sexes fulfilling the inclusion and exclusion criteria were included in the study. Then each patient was evaluated during each hemodialysis session for the (1) presence of bloodstream infection (BSI) using CDC (Centre for disease control) case definitions by blood cultures (Two samples were sent for cultures one from peripheral veins and another from vascular access either from fistula or from central venous catheter) and (2) clinical features. Result: The mean age of the patients were 38.26 (15.26) years. In this present study male (71.7%) are predominant than female (28.3%). Maximum (51.7%) patients were under weight. In this study 50 (83.3%) patients had anemia. Mean (SD) WBC, Hb, S. uric acid, FPG, Plasma glucose 2h ABF, HbA1C, S. total protein and S. albumin were 14415 (4000) count/cmm, 9.61 (1.62) g/dl, 417.67 (100.87) µmol/l, 5.37 (1.18) mmol/l, 7.27 (2.18) mmol/l, 6.42 % (0.68), 62.71 (5.82) g/l and 28.97 (4.99) g/l respectively. Out of 46 AV fistula cases, culture was positive in vein 8 (13.3%) cases and in fistula 5 (10.8%) cases. Out of 11 permanent CV catheter cases, culture was positive in vein 6 (54.5%) cases and in catheter