Grasscutters are currently being explored as laboratory models for scientific researches. The aim of the study was to Identify and localize Nissl bodies in neurons of the cervical spinal cord segments of grasscutters using cresyl fast violent (CFV) stains and to further measure the neuronal sizes of both anterior and posterior horns of the gray matter of the spinal cord. Six grasscutters comprising of three males and three females were utilized for the study. The studies were done using light microscopically methods. The animals were sacrificed by the method of decapitation and spinal cord was removed by splitting the vertebrae. The segments were fixed, processed, sectioned and stained with CFV for detailed studies of the spinal cord segments of the grasscutter. The result of the present study showed that the gray matter was stained dark purple due to the abundant presence of Nissl bodies in the neuronal cell bodies while the outer zone of white matter stained relatively lighter due to the presence of nerve fibers in the region. The result of the histometrical studies of cell areas in the anterior horn has the mean average of 2.56± 0.13 and the largest cell sizes of the cervical spinal cord was recordedin the anterior horn to be in 6.36± 0.16 while the posterior horn has the smallest cells with mean values of 3.86± 0.24. These findings can be useful in understanding the biology of the rodent.