Introduction: As we aim to cater high-quality health-care system to the masses with limited available resources, increased usage of generic medicines can improve affordability of the health care without compromising the quality. The attitude of healthcare providers is a significant challenge towards dealing with the rising healthcare cost. It is during internship that attitudes of future physicians towards prescribing are developed. Data regarding knowledge, attitude and practice of intern doctors for the use of generic medicines are lacking. Objectives: The study was conducted to assess knowledge, attitude and practice of intern doctors regarding use of generic medicines at a tertiary care teaching hospital. Materials and Methods: A questionnaire based cross sectional study was conducted after the approval of institutional ethics committee among the intern doctors at tertiary care teaching hospital, who were willing to respond to the study questionnaire were enrolled. The pre-validated questionnaire with 11 questions regarding knowledge, attitude and practice were sent to the participants via email. Responses were submitted online and data was analyzed in frequency and percentage.