Piper betle L. is an evergreen perennial dioecious creeper and it is native to Malaysia. It is commonly used in our day to day life. In present investigation an attempt has been made for the pharmacognostic standardization and phytochemical evaluation of Piper betle L. leaves. The pharmacognostic evaluation comprises of detailed morphology, powdered microscopy, quantitative microscopy and physical constants such as extractive and ash values. The leaves extract were subjected to preliminary phytochemical screening. The data obtained in present study will serve as valuable tool for identification, authentication and detection of adulterants standardization and quality control of the drug. In this research work the anthelmintic property of leaves extract of Piper betle L. belonging to family Piperaceae was studied for anthelmintic activity against Pheretima Posthuma (Indian earthworm). The leaves powder of Piper betle L. was subjected to successive extraction by maceration using solvents like Aqueous, Acetone + Aqueous and Pet. Ether. Four concentrations (25, 50, 75 and 100 mg / ml) of different extract of leaves were studied in a bioassay, which involved the determination of time of paralysis and time of death of the worms. 100 mg/ml conc. of Aqueous + Acetone extract of leaves of Piper betle L. reveal considerable anthelmintic activity as compared to other two concentrations and piperazine citrate (10 mg/ml). Piperazine citrate and saline water were included in the assay as standard reference drug and control, respectively.